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BCMB 204: Enzymology

Credits: 
2

Introduction to Enzymes: Comparison of chemical and enzyme catalysis, Activation energy and transition state, Free energy change, Chemical equilibria, Active site, Substrate specificity, Enzyme classification, enzyme assays, linked or coupled.
Factors affecting Enzyme Activity: Reaction rate (v), Effect of [S], [E], T, pH on enzyme activity; coenzyme, prosthetic groups.
Enzyme Kinetics and Inhibition: Michaelis - Menten model, Graphical representation of data (e.g. Lineweaver - Burk and Hanes plots)
Enzyme inhibition: Reversible (Competitive, noncompetitive, uncompetitive) and irreversible
Control of Enzyme Activity: Feedback regulation, allosteric enzymes, isozymes, covalent modification, activation, regulation of synthesis and breakdown (eg. lac operon, tryptophan biosynthesis).
Enzyme Purification: Cell disruption techniques, general purification strategy, enzyme assays, units of enzyme activity.
Application of enzymes in health, agriculture and industry