BCMB 200: Practical Biochemistry I

Acid-Base Reactions: Titration; pH measurement; buffer preparation; determination of pK.
Acid-base reactions; buffers, chromatography, qualitative analysis of carbohydrates, proteins and lipids. Quantitative analysis of proteins: methods for protein estimation (Folin-Lowry, Biuret, Ultraviolet absorption); determination of amino acids (ninhydrin method); preparation, purification and standardization of proteins (serum proteins, cytochrome C).
Separation Methods: Paper and gel electrophoresis; chromatography (Paper, TLC, column).
Quantitative analysis of carbohydrates: Estimation of glucose (Folin-Wu); isolation of glycogen, determination of rate of hydrolysis and chromatography of hydrolysis products.
Quantitative analysis of lipids: Solubility; emulsification; determination of iodine number and acid value; separation of serum lipids.