MLAB 417 : HISTOTECHNOLOGY III

Neurohistochemistry; Cellular Components of Nervous System; Nissl stains. Demonstration of Nerve Fibres; Demonstration of Myelin: Normal Myelin; degeneration products; combination techniques Demonstration of Neuroglial Cells; Demonstration of Nerve Endings; Immunohistochemistry; Definition of IHC; Paraffin sections and IHC; Unmasking Concealed Antigens; Reagents and antibodies for IHC (Labels: Enzymes, metals, radioactive materials); Chromogens and Substrates; Stability of Colour; Immunohistochemical methods; Reaction Product Intensification and Counterstains (Factors Influencing Immunohistochemistry Procedure, Application in Diagnostic Pathology); Immunofluorescence (Introduction, Fluorochromes, Staining Procedures: Direct and Indirect Staining;); Enzyme Histochemistry (Techniques for Demonstration of Enzymes).