MLAB 303 : BASIC CLINICAL AND LABORATORY HAEMATOLOGY

Haemopoeitic tissue and stem cells, haemoglobin formation from foetus to adults; Erythropoiesis, myelopoiesis, structure of red cell membrane, metabolism of red cell.; Function of red cells, white cell and platelets; Causes and effects of reduced and increased white cell and red cell count; Abnormalities of haemoglobin synthesis and catabolism.; Diagnosis and investigation of haemolytic anaemias. Parasitic infections in Haematology; The thick and thin peripheral blood film in diagnosis; Blood viscosity and erythrocyte sedimentation rate. Supravital staining; Granulopoiesis and lymphopoiesis, variations in the granulocyte and lymphocyte counts; The immune response; The immunology and biochemistry of phagocytosis; The structure and function of immunoglobulins; Lymphocyte subsets.; Principles of manual cell count and eosinophil count; Cell counting statistical applications SD, CV, Control chart Protein electrophoresis.; Hb electrophoresis and tests of function of red cell membrane. Study of HbS, HbF and Hg A2 Haem pigments. Assay of some red cell enzymes e.g. G6PD; Principles of assay of iron TIBC, ferritin, Vitamin B12, folic acid and the schilling test;