MLAB 213 : INTRODUCTION TO MOLECULAR BIOLOGY PRACTICAL

Extraction and quantitation of DNA and RNA; long term storage of nucleic acid; Restriction enzyme digestion; PCR; Agarose and polyacrylamide gel electrophoresis; Nucleic acid transfer to a membrane with subsequent prehybridization, hybridization, and stringency washes, probe labelling, autoradiography and sequencing. Basic bioinformatics applications. In silico analysis. DNA sequencing techniques. Cloning techniques. Introduction Micro array technology.