MLAB 204 : INTRODUCTION TO HAEMATOLOGY

Introduction (Definition and importance of specimens required and mode of collection, Use of syringes and needles of different sizes, lancets and vacutainer); Glassware (Slides, cover slip, beakers, measuring cylinders and pipettes, different flasks both glass and plastic); Equipment (The light microscope water bath incubators, weighing balances, centrifuges, fridges, colorimeters/spectrophotometers, cell counters (electronic and manual), auto pipettes, uses and maintenance; introduction to flow cytometry); Chemicals and Reagents (anticoagulants and preservatives in haematology, transfusion science; uses and preparation); Stains and staining (Introduction to Romanowsky stains. Thick and thin films); Preparation of solutions (Saline, buffers,metabisulphite solution, stock and working solutions (dilutions from stock) Haemoglobin estimation, sickling test, total WBC counts, ESR, reticulocyte count to be used as basis to elucidate above through demonstration and actual performance; ABO grouping as basis for particle agglutination.