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BCMB 407: CELL & MOLECULAR BIOLOGY PRACTICAL II

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Cell Biology:  Preparation of Cytoskeleton: Reactivation of Ciliary Beat; Cytoskeletal transformation e.g of sea urchins coelomocyles: Induction of Shape Change via Hypotonic Shock; Effect of Colchicine on Coelomocyte Cell Shape Changes; Effect of Ca2+ and Ca2+ ionophore on Shape Changes of Coelomocytes. Preparation of lymphocytes

DNA technology: Isolation, purification and manipulation of DNA; transformation and screening.