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Laud K. N-A. Okine (BSc (Ghana), PhD (Surrey))

Laud K. N-A. Okine (BSc (Ghana), PhD (Surrey))
Assoc. Professor (Contract)
lknokine@ug.edu.gh
6133

 

 

Training: Ph.D- Biochemical Toxicology, University of Surrey, UK.

 

Post-doctoral- Advanced Research Experience in Biochemical Toxicology, National Cancer Institute, Developmental Therapeutics Program, Division of Cancer Treatment, NIH, Bethesda, USA.

 

 

Approaches to Research:

 

Pre-clinical studies

  1. Preparation of crude extracts and organic fractions as well as isolation, purification and structural elucidation of biologically active chemical entities from selected organic fractions will be common to all plants/diseases.

  2. Studies on the determination of biological/pharmacological activity of extract and fractions, mechanism of action and molecular targets of selected chemical entities will depend on plant/disease of interest.

  3. In vitro cytotoxicity of crude extract, selected fractions and chemical entities, as well as safety evaluation of selected chemical entities in vivo in laboratory animals will be common to all plants/diseases.

  4. Pharmacokinetic, dose-response and drug interaction studies will be conducted with selective active chemical entities in rodents and/or dogs, which will be common to all plants/diseases.

  5. Selected chemical entities may be modified to produce more active counterparts, which will be tested as for points 2-4.

Clinical Studies

  1. Phases 1, 2, 3 and 4 will be conducted with selected chemical entity and its more active modified entity.

 

 

AREA(S) OF SPECIALIZATION/RESEARCH INTEREST(S)

 

  • Research and Development of herbal medicines as alternatives to allopathic medicines.

  • Research and Development of drug candidates from selected medicinal plants for the treatment/management of diabetes mellitus (DM), hypertension, malaria and trypanosomiasis.

  • Safety evaluation of drugs and herbal medicines.

  • Evaluation of the efficacy and mode of action of drugs and herbal medicines.

  • Development of in vitro methods for the rapid screening of efficacies and safety of herbal medicines.

  • Mechanisms of drug toxicity and resistance (use of animals/cell cultures).

 

PLANTS OF INTEREST

 

Annickia polycarpa and Securinega virosa (DM)

Vernonia amagdalina and Tetrapleura tetraptera (hypertension)

Polyanthia longifolia and Alstonia bonnei (malaria)

Morinda lucida and Zanthoxylum xanthoxyloides ( trypanosomiasis)

 

 

SELECTED PUBLICATIONS

 

  1. Takanori Hori, Jacob Barnor, Tung Nguyen Huu, Osamu Morinaga, Akiko Hamano, Jerry Ndzinu, Angela Frimpong, Keren Minta-Asare, Mildred Amoa-Bosompem, James Brandful, John Odoom, Joseph Bonney, Isaac Tuffour, Baffour-Awuah Owusu, Mark Ofosuhene, Philip Atchoglo, Maxwell Sakyiamah, Richard Adegle, Regina Appiah-Opong, William Ampofo, Kwadwo Koram, Alexander Nyarko, Laud Okine, et al.(2015). Procyanidin Trimer C1 Derived from Theobroma cacao Reactivates Latent Human Immunodeficiency Virus Type 1 Provirus. Biochem. Biophys. Res. Commun. 459: 288-293.

  2. Donkor, Kofi, Okine, Laud K.N-A., Abotsi, Wonder and Woode, Eric (2014). Acute and sub-chronic toxicity studies of aqueous extract of root bark of Cassia sieberiana D.C. in rodents. J. Appl. Pharm. Sci. 4(4): 84-89.

  3. Ocloo, A., Okpattah, W.E., Quasie, O., Sakyiamah, M.M. and Okine, L.K.N. (2014).Concurrent administration of aqueous extract of Cryptolepis sanguinolenta reduces the effectiveness of artesunate against Plasmodium berghei in rats. J. Appl. Pharm. Sci. 4(3): 24-28.

  4. Donkor, Kofi, Antwi, Stephen, Asiedu-Larbi, Jerry, Takyi, Nutifafa and Okine, Laud Kenneth (2014). Sub-chronic toxicity studies of Asena, a poly-herbal formulation for the treatment of arthritis in rat. Med. Arom. Plant Res. J. 2(1): 18-27.

  5. Asiedu-Larbi, J., Adjimani, J.P., Okine, L.K.N., Addy, M.E., Nyarko, A.K. and Nyarko, B.J.B. (2014). Efficacy studies on Mist Diodia, an herbal preparation for the management of hypertension in rodents. Med. Arom. Plant Res. J. 2(1): 6-17.

  6. Okine, L.K.N. and Yirenkyi, A. (2014). Standardization and scientific validation of herbal medicines. In: Molecular Approaches to Understanding Life and Disease-University of Ghana Readers, Physical & Life Sciences Series Vol. 4, Okine, L.K.N., Gbewonyo, W.S.K. and Nyako, M. (eds), Digibooks Ghana Ltd, Tema, Ghana, pp 81-101.

  7. Appiah, A A., Asomaning, W.A., Oppong, I.V., Duker-Eshun, G., Clement, J., Okine, L.K.N., Achel, G., Gyampo, O., Adjei, S., Nyarko A.K., Aboagye, F.A. and Edoh, D.A. (2013). Prospects of Croton membranaceus for Prostate Health. In: African Natural Products Volume II: Discoveries and Challenges in Chemistry, Health and Nutrition. ACS Symposium Series, Vol. 1127, pp 79-92.

  8. Donkor, K., Antwi, S., Asiedu-Larbi, J., Takyi, N., Martey, N.O. and Okine, L.K. (2013). Analgesic and anti-inflammatory activities of Asena, an herbal preparation for treatment of arthritis, using rodent models. Med. Arom. Plant Res. J. 1 (12): 20-29.

  9. Martey, O.N.K., Shittah-Bay, O., Owusu, J. and Okine, L.K.N. (2013). The antiplasmodial activity of an herbal antimalarial, AM-207 in Plasmodium berghei-infected Balb/c mice: Absence of organ specific toxicity. J. Med. Sci. 13 (7) 537-545.

  10. Martey, O.N.K., Armah, G.E., Sittie, A.A. and Okine, L.K.N. (2013).  A chronic toxicity study of the ground root bark of Capparis erythrocarpus(Cappareceae) in male Sprague-Dawley rats. Pak. J. Biol. Sc. 16 (23): 1706-1713.

  11. Kofi Donkor, Okine, L.N.K. Wonder K.M. Abotsi and Eric Woode (2013).  Anti-inflammatory and anti-nociceptive effects of ethyl acetate fraction of root bark of Cassia siberiana D.C. in murine models.  Pharmacologia 4 (4): 301-310.

  12. Ayivor, J.E., Okine, L.K.N., Dampare, S.B., Nyarko, B.J.B. and Debrah, S.K. (2012). Application of Westcott Formalism Ko NAA method to estimate short and medium lived elements in some Ghanaian herbal medicines complemented by AAS. Rad. Phys. Chem. 81: 403-409.

  13. Owiredu, W.K.B.A, Takyi, N.K., Okine, L.K.N., Sittie, A.A., Amidu, N., Boateng, K.A., Antwi, S., Donkor, K. and Addae-Mensah, O. (2011). Safety evaluation of BF001, an antidiabetic herbal preparation, in Sprague-Dawley rats. W. Afr. J. Pharm. 22(11): 49-57.

  14. Yirenkyi, A.M., Sittie, A.A., Okine, L.K.N. and Brew-Daniels, H. (2010). Development and standardization of dry ethanolic extract of roots ofCryptolepissanguinolenta (Lindl). Schlt. Ghana Pharm. J., pp 40-42.

  15. Martey, O.N.K., Armah, G.E. and Okine, L.K.N. (2010). Absence of organ specific toxicity in rats treated with Tonica, an aqueous herbal haematinic preparation. Afr. J. Trad. CAM. 7 (3): 231-240.

  16. Quasi, O., Martey, O.N.K., Nyarko, A.K., Gbewonyo, W.S.K. and Okine, L.K.N. (2010). Modulation of penile erection in rabbits by Mondia whitei: Possible mechanism of action. Afr. J. Trad. CAM. 7 (3): 241-252.

  17. Adusi-Poku, Yaw,Okine,L.K.N, Hlortsi-Akakpo, F. K., Fleischer, Theophilus C., Mensah, M. L. K., Arhin, Peter, Agyemfra, George, Dabra VI, Togbega, Mensah, E. N. (2010). Assesssing herbal medical practitioners in professional qualifying examination in Ghana, a model. Afr. J. Trad. CAM. 7 (1): 85-87.

  18. Martey, O.N.K., Ocloo, A., Koomson, E. and Okine, L.K.N. (2009). Assessment of Tonica, an aqueous herbal haematinic, in the modulation of rat hepatic microsomal CYP-mediated drug metabolizing enzymes: Implications for drug interactions. J. Pharmacol. Toxicol. 4(3): 70-78.

  19. Antwi, S., Martey, O.N.K., Donkor, K.N. and Okine, L.K.N. (2009). Anti-diarrhoeal activity of Blighia sapida (Sapindaceae) in rats and mice. J. Pharmacol. Toxicol. 4(3): 117-125.

  20. Nartey, E.T., Addo, P.G., Ofosuhene, M., Okine, L.K.N. and Nyarko, A.K. (2009). The effect of Cassia sieberiana root bark extract on various experimental gastric ulcer models in rats. J. Phytomed. Therap. 14: 55-61.

  21. Adamafio, N.A., Okine, L.K.N. and Adjimani, J.P. (2005). Integration and Control of Metabolism, iUniverse Publishing Inc., Lincoln, Nebraska.

  22. Nyarko, A.K., Okine, L.K.N., Wedzi, R.K., Addo, P.A. and Ofosuhene, M. (2005). Subchronic toxicity studies of the antidiabetic preparation ADD-199 in the rat: Absence of organ specific toxicity and modulation of cytochrome P-450. J. Ethnopharmacol. 97: 319-325.

  23. Okine, L.K.N., Nyarko, A.K., Oppong, I.V., Osei-Kwabena, N., Barnes, F. and Ofosuhene, M. (2005). The antidiabetic activity of the herbal preparation ADD-199 in mice: A comparative study with two oral hypoglycaemic drugs. J. Ethnopharmacol. 97: 31-38.