This advanced biochemistry course focuses on enzyme-catalyzed reactions and their practical applications, culminating in a hands-on mini-project. Students explore the kinetics of enzymatic reactions, examining how factors such as enzyme concentration, pH, temperature, substrate concentration, and the presence of activators or inhibitors affect reaction rates. The concept of enzyme specificity is thoroughly investigated. Practical exercises include studying protease activity in plant extracts and purifying enzymes from plant juice, providing real-world context to theoretical concepts. The course emphasizes the analytical applications of enzymes, such as the estimation of urea in urine. The highlight of the course is a mini-project where students isolate, purify, and characterize a known enzyme, offering a comprehensive, hands-on experience in enzyme biochemistry. This project integrates various techniques learned throughout the course, allowing students to apply their knowledge in a research-like setting. By combining theoretical understanding with practical skills, this course prepares students for advanced work in enzymology and biochemical research.